Curriculum Vitae of Dr. Diane Hammit


DIANE G. HAMMITT, B.S., M.S., PH.D., HCLD

Appointments:

Graduate School:
Research Assistant I; University of Illinois; Champaign, IL; 5/76 - 9/76
Graduate Research Assistant; Southern Illinois University; Carbondale, IL; 9/76 - 12/77
Graduate Teaching Assistant; Virginia State University; Blacksburg, VA; 8/79 - 12/80
Lab Director, Swine Genetics International; 1/81 - 12/82
Adjunct Instructor; Iowa State University, Ames, IA; 1/83 - 5/85

University of Iowa Hospitals and Clinics:
Director, In Vitro Fertilization (IVF) and Reproductive Testing Laboratories, University of Iowa Hospitals & Clinics, Iowa City, IA; 7/85 - 4/93
Associate Research Scientist; University of Iowa Hospitals & Clinics; Iowa City, IA; 7/85 - 7/90
Research Scientist; University of Iowa Hospitals & Clinics; Iowa City, IA; 7/90 - 4/93
Adjunct Associate Professor; University of Iowa Hospitals & Clinics; Iowa City, IA; 11/92 - 4/93

Mayo Clinic Rochester, Minnesota:
Temporary Consultant & Director, IVF and Andrology Laboratories, 11/92 - 3/93
Director, IVF and Andrology Laboratories, 4/93 - 12/01
Consultant, Division of REI, 1/02-12/01
Coordinator, Anonymous Oocyte Donation and Embryo Donation Programs, 1/1/95 - 6/30/97
Associate Professor; Mayo Medical School, 1/94 - 6/05

Mayo Clinic Scottsdale, Arizona:
4/96 – 6/30/05 Director, IVF and Andrology Labs
Co-Director IVF Program, 4/96 - 12/00
Coordinator, Anonymous Oocyte Donation Program, 4/96- 4/00
Associate Professor; Mayo Medical School, 1/94 - 6/05

Education:
Eastern Illinois University; Charleston, IL B.S. 1976
Southern Illinois University; Carbondale, IL M.S. 1978
Iowa State University; Ames, IA Ph.D. 1985


Board Certifications:
High Complexity Clinical Laboratory Director (HCLD), November, 1995.
American Board of Bioanalysis (ABB) certification exams:
-- General Knowledge 10/6/95, passed
-- Andrology 10/6/95, passed
-- Embryology 10/6/95, passed
-- Embryology Laboratory Director (ELD) Certification, November 2002

Awards and Honors:
Life Science Award for Academic Excellence; Eastern Illinois University; 1976
Nomination to Gamma Sigma Delta Honorary Society; Iowa State University; 1984
Nomination to Phi Kappa Phi Honorary Society; Iowa State University; 1984
Clinical and
Research Interests: Assisted Reproductive Technologies (ART)
Diagnosis and Treatment of Male-factor Infertility
Intracytoplasmic Sperm Injection (ICSI)
Preimplantation Genetic Diagnosis (PGD)
Assisted Hatching
Embryo and Oocyte Cryopreservation

Professional Memberships and Societies:
American Society for Reproductive Medicine (ASRM, previous AFS), 1984-present
- Editorial Board, Fertility and Sterility, 7/94-7/99
- Ad Hoc Reviewer, 1/87-12/00
Alpha International Society for Embryologists, 1/12/95 - present
American Society of Animal Science, 1981-1985
American Association for the Advancement of Science, 1984-1986
American Association of Bioanalysts, 1994 - present
International Embryo Transfer Society, 1984-1999
International Federation of Fertility Societies, 1984-present
Gamma Sigma Delta Honorary Society, 1984-1986
Phi Kappa Phi Honorary Society, 1984-1986
Society for the Study of Reproduction, 1985-1999
American Society of Andrology, 1985, 1987-present
- Editorial Board, J. Andrology, 11/94 - 12/97
American Association of Tissue Banks, 1989-2000
Reproductive Biology - Special Interest Group (ASRM Affiliated), 1989-present
Laboratory Technologists - Special Interest Group (ASRM Affiliated), 1991-present
Pacific Coast Fertility Society (ASRM Affiliated), 1992-present

Epididymal and Testicular Biopsy Sperm Preparation for IVF/ICSI
Zygote and Embryo Grading Systems to Decrease Risk of Multiple Pregnancy in IVF
Optimizing Methods for Culturing Human Embryos to the Blastocyst Stage


Peer-Review Publications:

Hodson HH, Hausler CL, Hammitt DG, Wilkens MA, Arthur RD: Effect of gonadotropin dose and postpartum status on induced ovulation and pregnancy in lactating sows. J Anim Sci 52:688, 1978.

Martin PA, Hammitt DG, Strohbehn RS, Keister DM: Induction of parturition in swine with Epostane, a competitive inhibitor of 3B-hydroxysteroid dehydrogenase. J Anim Sci 64:497, 1987.

Hammitt DG, Muench MM, Williamson RA: Antibody binding to greater than 50% of sperm at the tail tip does not impair male fertility. Fertil Steril 49:174, 1988.

Hammitt DG, Aschenbrenner DW, Williamson RA: Culture of cytomegalovirus from frozen-thawed semen. Fertil Steril 49:554, 1988.

Hammitt DG, Walker DL, Williamson RA: Concentration of glycerol required for optimal fertility of frozen semen is dependent on medium in which human sperm are frozen. Fertil Steril 49:680, 1988.

Martin PA, Hammitt DG, Strohbehn RS, Keister DM: Effect of Epostane, a competitive inhibitor of the 3B-hydroxysteroid dehydrogenase enzyme, in cyclic sows. Theriogenology 30:823, 1988.

Hammitt DG, Hade DK, Williamson RA: Survival of human sperm following controlled- and noncontrolled-rate freezing. Andrologia 21:311, 1989.

Hammitt DG, Martin PA: Fertility of frozen-thawed porcine semen following controlled-rate freezing in straws. Theriogenology 32:359, 1989.

Hammitt DG, Martin PA: Correlations among assays of porcine seminal quality following cryopreservation. Theriogenology 32:369. 1989.

Hammitt DG, Martin PA, Callanan T: Correlations between heterospermic fertility and assays of porcine seminal quality before and after cryopreservation. Theriogenology 32:385, 1989.

Hammitt DG, Bedia E, Rogers PR, Syrop CH, Donovan JF, Williamson RA: Comparison of motility stimulants for cryopreserved human semen. Fertil Steril 52:495, 1989.

Hammitt DG, Walker DL, Syrop CH: Improved methods for preparation of culture media for IVF and GIFT. Hum Reprod 5:457, 1990.

Hammitt DG, Walker DL, Williamson RA: Survival and in vitro fertilizing potential of sperm following washing and incubation with different protein supplements. Int J Fertil 35:46, 1990.

Hammitt DG, Syrop CH, Hahn S, Walker DL, Butkowski C, Donovan JF: Comparison of concurrent pregnancy rates for in vitro fertilization-embryo transfer (IVF-ET), pronuclear stage embryo transfer (PROST), and gamete intrafallopian transfer (GIFT). Hum Reprod 5:947, 1990.

Hammitt DG, Aschenbrenner DW, Heinkel DD, Syrop CH: Techniques for improving the accuracy and efficiency of the sperm penetration assay (SPA). Andrologia 23:11, 1991.

Hammitt DG, Walker DL, Syrop CH, Miller TM, Bennett MR: Treatment of severe male-factor infertility with high concentrations of motile sperm by micro-insemination in embryo cryopreservation straws. J In Vitro Fert Embryo Transf 8:101, 1991.

Hammitt DG, Rogers PR, Ruble KA, Syrop CH, Hood CC: Human sperm survival, two-cell zona free, and one-cell zona-intact assay for IVF quality control. Intl J Fertil 38:347-354, 1992.

Van Voorhis BJ, Gerard JL, Hammitt DG, Syrop CH: Use of oocytes from anonymous, matched, fertile donors for prevention of heritable genetic diseases. J Med Genetics 29(6):398-399, 1992.

Robinson D, Syrop CH, Hammitt DG: After superovulation-intrauterine insemination fails: The prognosis for treatment by gamete intrafallopian transfer/pronuclear stage transfer. Fertil Steril 57(3):606, 1992.

Van Voorhis BJ, Syrop CH, Hammitt DG, Dunn M, Snyder G: Effects of smoking on ovulation induction for assisted reproductive techniques. Fertil Steril 58:981, 985, 1992.

Hammitt DG, Syrop CH, Van Voorhis BJ, Walker DL, Miller TM, Barud KM, Hood CC: Prediction of nuclear maturity from cumulus-coronal morphology: Influence of embryologist experience. J Assist Reprod Genet 9:439-446, 1992.

Hammitt DG, Syrop CH, Van Voorhis BJ, Walker DL, Miller TM, Barud KM: Maturational asynchrony between oocyte cumulus-coronal morphology and nuclear maturity in GnRH-a stimulations. Fertil Steril 59(2):375, 1993.

Hammitt DG, Syrop CH, Walker DL, Bennett MR: Conditions of oocyte storage and use on noninseminated as compared with inseminated, nonfertilized oocytes for the hemizona assay (HZA). Fertil Steril 60(1):131-136, 1993.

Wenstrom KD, Syrop CH, Hammitt DG, VanVoorhis BJ. Increased risk of monochorionic twinning associated with assisted reproduction. Fertil Steril 60(3):510-514, 1993.

Hammitt DG, Rogers PR, Ruble KA, Syrop CH, Hood CC. The human sperm-survival, two-cell zona-free, and one-cell zona-intact assays for IVF quality control. Int. J. Fertil, 38(6):347-356, 1993

Lu PY, Hammitt DG, Zinsmeister AR, Dewald GW. Dual color fluorescence in situ hybridization to investigate aneuploidy in sperm from 33 normal males and a man with a t(2;4;8)(q23;q27;p21). Fertil Steril, 62:394, 1994.

Hammitt DG. Treatment of severe male-factor infertility by intracytoplasmic sperm injection. Postgraduate Obstetrics and Gynecology, 14(17): August 1994

Rhoton-Vlasak, Lu PY, Barud KM, Dewald GW, Hammitt DG. Efficacy of Calcium Ionophore A23187 Oocyte Activation for Generating Parthenotes for Human Embryo Research. J. Assisted Reprod Genetics 13(10):793-796, 1996.

Goudas, VT, Hammitt DG, Damario MA, Session DR, Singh AP, Dumesic DA. Blood on the embryo transfer catheter is associated with decreased rates of embryo implantation and pregnancy with the use of in vitro fertilization-embryo transfer. Fertility and Sterility 70(5):878-882, 1998.

Session DR, Hammitt DG, Damario MA, Dumesic DA. Recent Advances in Infertility Treatment. Minnesota Medicine 81:27-32,1998.

Damario MA, Hammitt DG, Galanits TM, Stevens SA, Session DR, Dumesic DA. Anonymous oocyte donation performed exclusively with embryos cryopreserved at the pronuclear stage. Fertility and Sterility 71(5):830-835, 1999.

Damario MA, Goudas VT, Session DR, Hammitt DG, Dumesic DA. Crinone 8% vaginal progesterone gel results in lower embryonic implantation efficiency following in vitro fertilization-embryo transfer. Fertility and Sterility 72(5):830-836,1999.

Damario MA, Hammitt DG, Galanits T.M., Session DR, Dumesic DA. Comparison of cryopreservation of supernumerary embryos at the pronuclear stage after intracytoplasmic sperm injection (ICSI) as compared to conventional in vitro fertilization (IVF). Fertility and Sterility 72(6):1049, 1999.

Damario MA, Hammitt DG, Session DR, Dumesic DA. Embryo cryopreservation at the pronuclear stage and efficient embryo use optimizes the chance for a liveborn infant from a single oocyte retrieval procedure. Fertility and Sterility, 73(4):767-773, 2000.

Damario MA, Hammitt DG. Maximizing IVF-ET efficiency and outcome through embryo cryopreservation. Reproductive Technologies 10(2): 86-92, 2000.

Hammitt DG, Ferrigni RG, Sattler CA, Rebert JA, Singh AP. Development of a new and efficient laboratory method for processing testicular sperm. J. Assisted Reproduction and Genetics 19(7):313-322, 2002.

Walker DL, Hammitt DG, Dumesic PA, Thornhill AR. Equivalent blastocyst rates after freezing murine embryos in Cryo Bio System high security or standard Instruments-Medicine-Veterinarian straws. Fertility and Sterility, 80, Suppl. 2: 743-746, 2003

Walker DL, Tummon IS, Hammitt DG, Session DR, Dumesic DA, Thornhill AR. Vitrification versus programmable rate freezing of late stage murine embryos: a randomized comparison prior to application in clinical IVF. Reprod BioMed Online:8(5):558-68, 2004.

Hammitt DG, Sattler CA, Manes ML, Singh AP. Selection of Embryos for Day-3 Transfer at the Pronuclear-Stage and Pronuclear-Stage Cryopreservation Results in High Delivery Rates in Fresh and Frozen Cycles and Optimizes Deliveries per Retrieval. J. Assisted Reprod. Genetics 21(7):271-278, 2004.

Book Chapters:

Hammitt DG: Treatment of male-factor infertility by in vitro insemination with high concentrations of motile sperm. Seminars in Reproductive Endocrinology 11(1):72-82, 1993 (Invited Chapter).

Non-Peer-Review Publications:

Hodson HH, Hammitt DG: New techniques in swine artificial insemination. Proc Minn Vet Assoc, 1983.

Hammitt DG, Syrop C, Hahn S, Walker D, Butkowski C, Donovan J: Clinical results of the in vitro fertilization (IVF) and gamete intrafallopian (GIFT) program at the University of Iowa Hospitals and Clinics, Iowa City, Iowa.
J In Vitro Fert Embryo Transf 6:316, 1989.

Hammitt DG, Williamson RA: Screening for cytomegalovirus antibody. Letter to the Editor. Fertil Steril 53(1):185, 1990.

Hammitt DG: Preparation of IVF media and bioassays for gamete and embryo toxins. In: Reproductive Biology: Clinical and Basic Perspectives. American Fertility Society 1991 Postgraduate Course (Invited Speaker).

Hammitt DG: Design of the IVF laboratory, contamination control, and laboratory bio- and chemical-hazardous waste disposal. In: Reproductive Biology: Clinical and Basic Perspectives. American Fertility Society 1991 Postgraduate Course (Invited Speaker).

Erickson, L.D. and Hammitt DG: New Treatments for Male Factor Infertility. Prime Practice vol. 2, number 1, 1996 (CD-ROM).

Chezick P, Dumesic DA, Hammitt DG, Hanson MA, Hicks GH, McConnel JP, Wentworth MA. Infertility - Where Do You Start? Mayo Medical Laboratories Communique 24(6):1-5, 1999.

Damario MA, Hammitt DG, Session DR, Dumesic DA. The most interesting studies are those that don't turn out the way you expected. Neils Bohr - Reply Fertility & Sterility 73 (5): 1068-9, 2000.

Debenport R, Eichenberger L, Hammitt DG, Hunter K, Kemman S, Singh A, Stropp J. Patient Financial Services Center for Reproductive Medicine Partnership. Development of the A/R-CRM Database. Mayo Clinic Scottsdale Innovation Week-A Celebration of the MCS Strategic Plan. Nov. 18-22, 2002, Scottsdale, Arizona.

Published Abstracts:

Hammitt DG, Snyder HH, Hausler CL: Pregnant mare serum and human chorionic gonadotropin for inducing ovulatioon in lactating sows. Annual Meeting Abstracts, J Anim Sci, 1978.

Hahn SJ, Holz L, Hammitt DG: Prospective data collection in a program for intrauterine insemination with washed sperm. Proc Iowa Acad Aci 94:157, 1987.

Syrop CH, Deidre R, Hammitt DG: Failure of superovulation - intrauterine insemination does noot affect the success of subsequent gamete intrafallopian transfer/pronuclear stage transfer. Society for Gynecologic Investigation, 1990.

Follett KL, Hammitt DG, Bennett MR, Syrop CH: Enhanced development of human embryos cocultured with vero cells. American Fertility Society Annual Meeting, October 19-24, 1991.

Voorhis BJ, Snyder GD, Hammitt DG, Syrop CH: Effect of cigarette smoking on intrafollicular steroid levels. American Fertility Society Annual Meeting, October 19-24, 1991.

Vincent RD, Hammitt DG, Baker M, Wegrzynowicz E, Rogers PR, Oberholser J: Evaluation of the potential embryo toxicity of propofol using an in vitro fertilization quality control test (hamster sperm motility assay). Annual Meeting of the Society for Obstetric Anesthesia and Perinatology, May 10, 1992.

Lu PY, Zinsmeister AR, Hammitt DG, Dewald GW. Dual color fluorescence in situ hybridization (FISH) of human sperm in 21 normal males and a male with 46,XY, t(2;4;8) (q23;q27;p21) translocation. Society for Gynecologic Investigation Meeting Abstracts, March 1994.

Bluestein DL, Zinsmeister AR, Hammitt DG, Dewald GW. The use of fluorescent in situ hybridization to assess the percoll density gradient and swim-up preparation in separating human X- and Y- chromosome bearing sperm. South Central Section of the American Urological Association, August 27-31, 1994.

Lu PY, Hammitt DG, Stropp J, Stevens S, Tri M, and Ory SJ. Optimal scheduling of controlled ovarian hyperstimulation (COH) avoids weekend oocyte retrievals for in vitro fertilization (IVF). 1995 Annual Meeting of ASRM, October 7-12, 1995.

Lu PY, Dewald GW, Ory SJ, Walker DL, and Hammitt DG. Fluorescence in situ hybridization (FISH) and cytogenetic analysis of unfertilized oocytes after intracytoplasmic sperm injection (ICSI). 52nd Annual Meeting of ASRM, October 7-12, 1995.

Rhoton-Vlasak A, Lu PY, Ory SJ, Dewald GW, Hammitt DG. Usefuleness of calcium ionophore A23187 oocyte activation for human embryo research. Society for Gynecologic Investigation (SGI) Annual Meeting, March 1996

Lu PY, Dewald GW, Ory SJ, Walker DL, and Hammitt DG. Fluorescence in situ hybridization (FISH) and cytogenetic analysis of unfertilized oocytes after intracytoplasmic sperm injection (ICSI). Society for Gynecologic Investigation (SGI) Vol. 3, No. 2 (supplement), Mar/April, 1996.

Nehra A, Hammitt DG, Harmon WJ, Erickson LD, Walker D, Miller T, Barud K, Dumesic DA: Intracytoplasmic Sperm Injection for Male Factor Infertility. Submitted for the North Central Urologic Association, October 22-November 2, 1996

Damario MA., Hammitt DG, Stevens SA, Session DR, Dumesic DA. Anonymous oocyte donation performed exclusively with embryos cryopreserved at the pronuclear stage. ESHRE Annual Meeting, May, 1998.

Damario MA, Hammitt DG, Singh A, Session DR, Dumesic DA. Pronuclear embryo cryopreservation without cleavage-stage embryo preselection permits high oocyte retrieval-specific cryoaugmented pregnancy rates. 1998 IFFS/American Society for Reproductive Medicine Meeting. San Francisco, California. P-045 (S139).

Hammitt DG, Nehra A, Damario M, Galanits T, Barud K, Dumesic DA. Cryopreservation of all extra sperm and embryos results in high cumulative pregnancy rates in an epididymal aspiration program. 1998 IFFS/American Society for Reproductive Medicine Meeting. San Francisco, California. P-643 (S328).

Hammitt DG, Walker DL, Sattler CA, Damario MA, Singh AP, Dumesic DA. Development of a homologous cumulus coculture system for IVF. 1998 IFFS/American Society for Reproductive Medicine Meeting, San Francisco, California.
O-259 (S97).

Damario MA, Hammitt DG, Galanits T.M., Session DR, Dumesic DA. Cryopreservation of supernumerary pronuclear stage human oocytes after intracytoplasmic sperm injection (ICSI) as compared to conventional in vitro fertilization (IVF). ESHRE Annual Meeting, May, 1999, Sydney Australia. Selected for oral presentation.

Damario, MA, Hammit, DG, Session, DR, Dumesic, DA. Embryo cryopreservation at the pronuclear stage and efficient embryo utilization optimizes the chance for a liveborn from a single oocyte retrieval. ASRM Annual Meeting, September, 1999, Toronto Canada. Selected for poster presentation.

Damario, MA, Hammitt, DG, Stevens, SA, Session, DR, Dumesic, DA. Anonymous oocyte donation performed exclusively with embryos cryopreserved at the pronuclear stage: an extended series. ASRM Annual Meeting, September, 1999, Toronto, Canada. Selected for poster presentation.

Hammitt, DG, Barud, KM, Galanits, TM, Wentworth, MA, Walker, DL, Damario, MA, Singh, AP, Dumesic, DA. Comparison of short and long sperm-oocyte coincubation in organ culture dishes (OCD) and tubes (TU). ASRM Annual Meeting, September, 1999, Toronto, Canada. Selected for oral presentation.

Hammitt, DG, Singh, AP, Walker, DL, Sattler, CA, Galanits, TM, Barud, KM, Wentworth, MA, Damario, MA, Dumesic, DA. Accuracy of embryo preselection at pronuclear (PN) stage. 1999 ASRM Annual Meeting, Toronto, Canada. Selected for poster presentation.

Hammitt DG, Walker DL, Sattler CA, Singh AP. Comparison of monolayer and fibroblastoid formation in cumulus cultures following incubation with various types of IVF media. Submitted ASRM Annual Meeting, October, 2000.

Hammitt DG, Barud KM, Galanits TM, Wentworth MA, Damario MA, Session DR. Post-ICSI culture of oocytes in G1.2 versus HTF-SSS-fertilization, embryo quality and pregnancy outcomes. ASRM Annual Meeting, October, 2000.

Hammitt DG, Galanits TM, Walker DL, Singh AP, Damario MA, Session DR. Embryo quality and blastomere number following embryo coculture on cumulus monolayers versus standard culture. Submitted ASRM Annual Meeting, October, 2000.

Famuyide AO, Session DR, Damario MA, Thornhill AR, Hammitt DG, Dumesic DA. 3-Dimensional Transvaginal Ultrasonography for Ovarian Follicle Number Predicts Number of Mature Oocytes Retrieved During IVF. Oral presentation, American Institute of Ultrasound in Medicine, 45th Annual convention, March 11-14, 2001, Orlando, Florida, J Ultrasound Med 20:S1-S154 2001[S:68 No.1515].

Damario, MA, Hammitt, DG, Thornhill AR, Stevens, SA, Session, DR, Dumesic, DA. Long-term results of an oocyte donation program which utilizes cryopreserved embryos exclusively. 2001 ASRM Annual Meeting, Orlando, Florida. Selected for poster presentation.

Hammitt DG, Sattler CA, Ferrigni RG, Singh AP. Development of a new and efficient laboratory method for processing testicular sperm. ASRM Annual Meeting, October, 2001, Orlando, Florida. Selected for poster presentation.

Singh, AP, Sattler CA, Hammitt DG. Presence of cervical mucus at embryo transfer and IVF outcome. ASRM Annual Meeting, October, 2001, Orlando, Florida. Selected for poster presentation.

Singh, AP, Sattler CA, Hammitt DG. Novel frozen embryo transfer approach involving pronuclear stage cryopreservation and blastocyst transfer. ASRM Annual Meeting, October, 2001, Orlando, Florida. Selected for oral presentation.

Walker DL, Hammitt DG, Dumesic PA, Thornhill AR. Equivalent blastocyst rates after freezing murine embryos in standard IMV or CBSTM high security straws. Accepted, for ESHRE presentation, 2002.

Hammitt DG, Sattler CA, Rebert JA, Singh AP. A cost saving method for freezing human semen. 2002 ASRM Annual Meeting, Seattle, Washington.

Hammitt DG, Sattler CA, Rebert JA, Peat PB, Singh AP. An alternate approach to the culture and cryopreservation of human embryos to maximize IVF pregnancy rates per oocyte retrieval. PCRS Annual Meeting, April 23-27, 2003, Rancho Mirage, California. Selected for Oral Presentation and Workshop Conference.

Walker DL, Hammitt DG, Session DR, Tummon IS, Dumesic, DA, Thornhill AR. Randomized comparison of murine blastocyst cryopreservation methods. 59th ASRM Annual Meeting, San Antonio, Texas. October 11-15, 2003. Abstract #O-165.


Walker D.L., Hammitt D.G., Session D.R., Tummon I.S., Dumesic D.A. and Thornhill A.R. Blastocyst cryopreservation: a comparison of programmable rate freezing and vitrification. American Association of Bioanalysts Meeting, Las Vegas, NV, May 13-15, 2004.